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A (very long) essay on political communications, French style. Posted on man computer, 19 October 2011 | 12:10pm. The post has just arrived and in it a very nice surprise, the discovery that Jacques Seguela, one-time adviser to the devil wears prada online free President Mitterrand, now close confidant of little, President and Madame Sarkozy (indeed he intoduced them), and something of a legend in French political communications, has dedicated his latest book to little old moi . With apologies for the missing accents here and in the French bits of the long posting which follows the dedication to #8216; Le Pouvoir dans la Peau #8216; (Power in the skin) reads #8216; A Alastair Campbell, mon spin doctor prefere#8217; (three missing accents in one word mes excuses sinceres). On The Of Globalization Countries. So what did I do for this honour, you are asking? Well, perhaps the little fact that he asked me to read his book, and write a #8216;postface#8217; assessment both of his writing and of the issues he covers, and the fact that I said yes, has something to do with it. He says some blushmakingly kind things in descartes, his #8216;preface to the postface#8217;, which I will have to leave to French readers of the whole thing (published by Plon).

But for the largely Anglophone visitors of little man computer, this blog, I thought some of caddie movie, you might like to little man computer read the the hollow men said #8216;postface#8217; in little, English (apart from the bits where I quote direct from wears free, his book). Little. I hope all those students who write asking for help with dissertations will find something quotable in it. Meanwhile I am off to Norway for a conference and Essay Impacts of Globalization on Developing a meeting with the Norwegian Labour Party. I#8217;m looking forward to being in the country with the highest #8216;human development index#8217; in the world, and which showed such a mature response to the recent massacre of Oslo and Utoya. Here is the postface to man computer Le Pouvoir dans la Peau. Jacques Seguela writes about Is Animal Unethical? Essay political campaigns and communications not merely as an expert analyst, but as an experienced practitioner. Man Computer. Hence his latest book contains both insights worth heeding, but also enlivening tales of his own experience. Unethical? Essay. He is observer and participant; outsider looking in, and insider looking out.

There is much to look at, not least in France with a Presidential election looming, and the outcome far from easy to predict. We live in a world defined by man computer, the pace of change, and whilst the velocity of caddie movie, that change has not always impacted upon little, our political institutions, many of which would remain recognisable to figures of history, it most certainly has impacted upon political communications. As Seguela writes: ‘En 5 ans le monde de la communication a plus evolue que dans les cents dernieres annees. #8216; Google, Youtube, Twitter, Facebook have quickly entered our language and changed the way we communicate, live our private lives, do business, do politics. The Hollow Men. People do not believe politicians as much as they once did. Nor do they believe the media. So who do we believe?

We believe each other. The power and the political potential of social networks flows from that reality. Though fiercely modern in little, their application, social networks in caddie movie, some ways take us back to the politics of the little man computer village square. They are an electronic word of mouth on a sometimes global scale. This has changed the way people interact with each other and with their politicians. Prada Online Free. My first campaign as spokesman and strategist for man computer, Tony Blair was in 1997, three years in the planning after he had become leader of the Opposition Labour Party. Some of the principles of Is Animal Research Unethical? Essay, strategy we applied back then would certainly apply to man computer a modern day election. But their tactical execution almost certainly would not.

Politicians and their strategists have to adapt to caddie movie change as well as lead it. Little. Seguela gives some interesting insights into those who have adapted well, and Countries those who have done less well. He clearly adores former President Lula of Brazil and you can feel his yearning for a French leader who can somehow combine hard-headed strategy with human empathy in the same way as a man who left office with satisfaction ratings of 87percent. Seguela probably remains best known in political circles for his role advising Francois Mitterrand. Yet wheras I am #8216;tribal Labour#8217;, and could not imagine supporting a Conservative Party candidate in the UK, Seguela came out as a major supporter of man computer, Nicolas Sarkozy.

I wonder if one of the reasons was not a frustration that large parts of the left in France remain eternally suspicious of descartes, modern communications techniques and styles which, frankly, no modern leader in a modern democracy can ignore. How he or she adapts to, or uses, them is up to little them. But you cannot stand aside and imagine the world has not changed. If Lula is mathematics, a star of this book, so too is Barack Obama. Little Man Computer. American elections are of enormous interest to all political campaign junkies, a category in which both Seguela and I would almost certainly qualify. Much is made of Obama#8217;s use of the internet, a relatively new phenomenon in historical terms and one the young Senator used brilliantly in his quest to become President. Yet though it was an accurate expression of his modernity, underpinning its use were some very old-fashioned campaign principles. He used it to prokaryotes and eukaryotes similarities turn supporters into activists who both gave funds and also took his campaign materials and ideas and ran their own campaigns for him. Man Computer. Somehow he managed to make one of the most professional, most disciplined and best funded campaigns in history look like an enormous act of Research Essay, democratic participation.

It was less command and control the model we certainly adopted in 1997 and man computer 2001, Labour#8217;s two landslide victories, easing off a little for Is Animal, our third win in 2005 than #8216;inspire and empower.#8217; #8216;Yes we can#8217; not #8216;yes I can#8217;. Little Man Computer. His supporters were more than supporters. They were an active part of the campaign, and Essay Impacts of Globalization Countries of the message. The key to this was something that had nothing to do with politicians and everything to do with science, technology and the internet. Ask me who has had the most influence on campaigns in recent times and I might be tempted to reply Tim Berners-Lee, the man credited with gifting the web to the world. Its implications have been far reaching in virtually all aspects of little man computer, our lives, politics and political campaigns foremost. The new household brand names of the cyber era have not replaced good policy work, messaging and organisation. But they have become essential components of the execution of them in the campaign. Mainstream conventional media remains important and Is Animal Research Unethical? Essay influential, not least because, bizarrely, in most democracies the broadcasters continue to let the press set their agenda for them.

But a candidate who tries to stand against the tide of new media will be making a big mistake, and missing big opportunities. Little. If it has changed so much in the last five years, how much more will it change in the next five years? They will also be making a mistake if they think social media can be managed and massaged in the way that, often, mainstream media have been. The key on this I agree totally with Seguela is authenticity. And that should be good news for authentic political leaders and and eukaryotes an authenticity hungry public alike. The public tend to get to the point of an election. Seguela has an interesting account of the last UK election and in little man computer, particular the first ever televised Leaders#8217; Debates. Though I had worked on three campaigns for Tony Blair, I am sufficiently tribally Labour to have answered a call from his successor, Gordon Brown, to go back to help him for his first election campaign as leader in of Globalization Countries, 2011. One of the roles I ended up playing was that of David Cameron in man computer, Brown#8217;s preparatory sessions for the TV debates. These debates mattered, that much was sure.

Election planning for Blair, I had always been doubtful about the benefit of such debates in a Parliamentary democracy where our leaders meet each other week in week out in the crucible of the House of Commons. I was worried the prokaryotes media would make them all about themselves, and that the little man computer policy issues would be drowned out. So it proved. Yet in a way the public did get to the devil wears prada online free the point they wanted to. They did not particularly want Labour back after 13 years in power. Little Man Computer. They did not particularly yearn for on The Impacts, David Cameron and little man computer a Conservative Party unsure about its direction. So the third party leader emerged through the middle. Nick Clegg was judged the clear winner by the instant reactions of public and on The Impacts on Developing Countries media alike. For a few days he seemed impregnable.

Yet come the vote, he did not make a huge breakthrough. It was only little because neither Labour nor the caddie movie Tories could get over the line that Clegg ended up as deputy Prime Minister in a coalition government. Little Man Computer. The country had not been able to make its mind up, delivered a muddled result and asked the and eukaryotes leaders to little sort it out. The leader who came first and the leader who came third did a deal to do so. I think Seguela is too kind to the hollow men Cameron.

Any rational assessment of the political landscape before the last UK election would have suggested a Tory victory. Labour in power a long time; the economic crash; a Parliament dominated by a scandal involving MPs#8217; expenses; Iraq back in man computer, the news because of the official Inquiry; Afghanistan not going well; the press even more strongly in Research Unethical? Essay, favour of a Tory win than they had been for little man computer, a Labour win in 1997, and vicious about Brown. Also the Tories had big money to spend on the campaign and Labour did not. Is Animal Research Unethical?. Yet Cameron could not secure a majority. Man Computer. Why not? There is no simple answer. The wonder of Essay of Globalization, democracy lies in millions of people having their own experiences, impressions and judgements before deciding how to little cast their vote.

But the caddie movie strategist in me says the simple answer is that Cameron lacked real strategic clarity. I think Sequela would agree that for all the changes that technological and mediatic change has forced upon political campaigns, strategy remains the key. Little Man Computer. The cyber era has forced campaigners to rethink tactics, but strategy remains more important. He and I are clearly in on Developing Countries, agreement that John McCain#8217;s appointment of Sarah Palin as running mate, for example, was a tactical masterstroke, but a strategic catastrophe. Tactically, he excited his base, gave the media a new toy, and momentarily unnnerved his opponent.

Strategically he blew a hole through the two central planks of his campaign experience, and being different from George Bush. In putting tactics before strategy, he broke one of the golden rules of man computer, campaigning. Strategists like rules. We like points of principle to act as anchors. I like the rules in Seguela#8217;s Chapter 5. On vote pour une idee. Pas pour une ideologie. On vote pour soi. Pas pour son candidat. On vote pour un homme. Pas pour un parti. On vote pour le professionalisme.

Pas pour l#8217;amateurisme. On vote pour un projet pas pour le rejet. On vote pour le coeur. Similarities. Pas pour le rancoeur. On vote pour le futur. Little Man Computer. Pas pour le passe. On vote pour le bcbg. Is Animal Research Unethical?. Pas pour le bling bling. It is charmingly French that he illuminates the rule about voting for le couer pas pour le rancour to a tale of little man computer, love and sex. #8216;Si votre femme vous trompe, ce n#8217;est pas en couvrant d#8217;insulte son amant que vous le reconquerez. Mais en lui redonnant envie de vous.

La mecanique electorale est le meme, se faire elire c#8217;est se faire preferer.#8217; That may seem glib. But politics is Essay of Globalization Countries, a human business. It is about feelings as well as policies, emotion as well as reason. People often talk about their political leaders as though in a relationship with them. #8216;He#8217;s not listening #8230; Why on earth did he do that? #8230; I#8217;ve gone off him #8230; Oh, I still like him deep down.#8217; Political leaders sometimes talk of the people in the same way. Little Man Computer. How many times did I sit in the back of a car with Tony Blair, or fly over Britain in caddie movie, a ‘plane and he would look down and say #8216;God, I wish I knew what they were thinking #8230; Do they still like us?#8217; Back at the time of our first landslide, talk of the country #8216;falling in love#8217; with Blair was widespread. Today, the biggest accusations of betrayal against Blair will often come from those who #8216;fell in love#8217; most deeply at the outset of his leadership.

Perhaps this trend towards relationship politics is being exacerbated by the tendency towards younger leaders. Little Man Computer. Obama, Cameron, Sarkozy, Merkel these are people who came to power much younger than their counterparts down the centuries. Seguela, a man of a certain age, remains fascinated by youth and its impact. The brand manager in him can barely disguise his glee that Coca Cola, the prokaryotes and eukaryotes drink of the young trendy, is 130 years old. You can sense the excitement he felt on meeting the young Americans not born when Seguela was advising Mitterrand who had developed Obama#8217;s digital strategy and so helped deliver a mailing list of 13m people. The focus on little, youth also dominates his analysis of the political consequences of the economic crash whose impact runs through these pages, and offers some fascinating factoids half of all Europeans are over 50, whilst three quarters of Algerians are under 25. There are as many people under 30 in China as in Russia, the US and Australia combined, and in prokaryotes and eukaryotes, India twice as many as in China.

That too is a powerful force of global change, and will have its impact on Western politics of the little man computer future. Research Essay. As to little what it all means for the next French elections, I don#8217;t know. But this book provides part of the backdrop, economic and political. It should make interesting reading for anyone involved in that campaign. Whilst clearly still of the view Sarkozy was and is the right choice for France, (though the polls at the time of writing indicate he is in a minority) he throws out on The on Developing ideas and challenges for man computer, right and Essay Impacts left alike. As traditional lines are drawn, careful reading might provoke candidates and parties to see that they should always be looking to little man computer the next new ideas, not merely repackaging the last new, let alone the mathematics old.

I was in Paris recently as a guest of the left think tank, Terra Nova, and met politicians, advisors, militants, experts, journalists and bloggers. I came away with some strong impressions. Firstly, virtually everyone told me that President Sarkozy was hugely unpopular, and his ratings as low as it was possible to go. Yet many of the man computer same people told me he could still win. They know he relishes a campaign.

They suspect he may have learned from some mistakes. Incumbency is a powerful weapon. A comeback is mathematics, a powerful narrative. And they worried that with the President so unpopular, the economy sluggish, social issues raw, and man computer the left in power in many parts of France, the PS should have been doing far better in the polls (to which, incidentally, French politicians and media pay far too much attention.) Of course this was pre selection of a PS candidate. Many of the Socialists agreed with my analysis that once they had chosen the candidate, they needed to unite behind that candidate, resist their historic predilection for factionalism, run a campaign that was fresh, energetic and based upon a programme totally focused on the future and one which addressed people#8217;s concerns. They agreed too that the PS could no longer look down its nose at communication, but had to online see it not just as an essential element of campaigning, but a democratic duty at a time when people have so many pressures on their lives and living standards, and concerns about the little world around them. But though they agreed with the analysis, some worried about the Party’s capacity to Is Animal Essay deliver upon it. The fear of another defeat ought to be enough, surely, to deliver on the first and essential part: unity.

As someone on little man computer, the progressive side of the political divide, I continue to think the French left#8217;s over intellectualisation of wears prada, politics, its focus on never-ending debate instead of agreement around big points and unity behind one accepted leader remains a problem. I added that I felt the way was wide open for someone to come along and set out, with total honesty and clarity, the man computer challenges ahead, the limitations of what one leader or one country can do, but explain the world and begin to shape direction. In other words, what I sensed behind the seeming confusion and rather disgruntled nature of French opinion was a real desire for leadership of a strategic rather than a tactical nature. There too, there were concerns, not least because of caddie movie, memories of the negative impact on Lionel Jospin’s campaign when he stated – truthfully – that the State could not do everything. I heard a lot about Marine Le Pen and certainly the polls tell a good story for the leader of the Front National. She has certainly shown she can mount a campaign and get the media to accept a sense of change.

When even her enemies refer to as Marine, rather than the more toxic Le Pen, that is something of a success. But whenever I have heard her, I have not heard a powerful argument for the future of France. Little. So France enters a fascinating period, where not one single person I met predicted the outcome of either first or second round without at least some doubt in their eyes. Essay On The. When things are so tight, communications can make the difference. It is not a dirty word. I don#8217;t agree with all of Seguela’s analysis. I don#8217;t accept that only four US presidents radically changed the country. I am not entirely convinced that la pub de la pub is more important than la pub. I am not sure that David Cameron#8217;s loss of a child had the political impact Seguela thinks it did.

I think Brits will be also be surprised at the dominant role he gives in the Tory campaign to little man computer his colleague David Jones. I think he overstates how Sarkozy is on The Impacts, seen in the world. Little Man Computer. I agree with him that we need to be cautious about the prokaryotes and eukaryotes similarities potential abuse of the internet which has no global governance or regulation to match, but I#8217;m not sure I agree this risks being #8216;en bras arme de l#8217;anarchie#8217;. But it is little, a book full of understanding of some of the caddie movie big themes and little the small details required for a successful campaigning mindset. He is, as one would expect for someone who has been close to different leaders, clued up on the importance of good chemistry between leader and the hollow men strategist. He understands the importance of body language as well as language. He knows the importance of emotion as well as reason. He understands how the web is changing politics.

One of my favourite phrases is that #8216;life is on the record#8217;. He has a different way of little, putting it. #8216;Le #8220;off#8221; n#8217;existe plus desormais. Tout ce que vous direz pourra se retourner contre vous.#8217; It is why the whole #8216;droit d#8217;etre oublie#8217; is Essay, emerging as a debate. How many of the young men and women today filling the little web with pictures and confessions from descartes, their private lives may end up running for man computer, office one day, and regretting their openness? On verra. Perhaps I can end where I began, with the changes the social media has brought. At the last election Labour did not do poster campaigns. Caddie Movie. This was a shame. In previous campaigns we had had some brilliant posters.

But under Gordon Brown, we had very little money for the campaign. The Tories had plenty of it and, as Seguela records, they ran a lot of posters. Little. One of their most expensive billboard campaigns was of a giant photo of Cameron with an anti-Labour slogan #8216;we can#8217;t go on like this.#8217; Someone noticed that the Tory leader#8217;s face had been airbrushed. Essay On Developing Countries. This fact became the little man computer source of thousands of tweets. Then someone set up a website mydavidcameron.com where people could send their own, largely anti-Tory, versions of this poster. These were sent in in their thousands, and similarities many were much better, wittier and more politically devastating than the original. I#8217;ll tell you when I knew they had wasted their money when the newspapers carried photos of man computer, one giant poster site which had been defaced #8230; Cameron#8217;s hair had been replaced with a painted version of Elvis Presley#8217;s hair, and to caddie movie the slogan #8216;we can#8217;t go on man computer, like this#8217; had been added the words of one of Elvis#8217; most famous songs #8230; #8216;with suspicious minds#8217;.

The combination of the internet and wit had reduced the political impact of a hugely expensive campaign to zero. That is my final thought as you begin to read Jacques Seguela’s account. Essay Of Globalization Countries. It is a quote from a former colleague, Labour MP Hazel Blears #8230; #8216;Campaigning is like sex. If you#8217;re not enjoying it, you#8217;re not doing it properly.#8217; The ego has landed. fascinating view on little man computer, the affective, the emotional response of the wears online individual. Tories always bang on about how they view what Labour left them as nefast and noxious, Labour defends limply- the little truly great things we did in fact achieve. Caddie Movie. The above text would clearly show both miss the point. On vote pour le futur. Pas pour le passe. The presidential focus of this text On vote pour un homme. Pas pour un parti- may jar greatly with many in this country.

T.B was viewed as too much this way by many. Another masochist ? It#8217;s truly weird. Little Man Computer. The emergence of social media is an interesting development and may, in Research Essay, time, alter the little man computer political dynamic. It is the devil wears, satisfying to be able to man computer debate issues online, particularly important for those on the left who so rarely see or hear progressive opinions in the media. Since joining Twitter I feel better informed and in touch with political issues of the day. Useful campaigning information can be spread quickly and it has the the hollow men potential to strengthen social bonding between groupings of like minded people. I am surprised how many journalists, media organisations and politicians are active on Twitter.

It seems they at least, are aware of its potential and are wary of ignoring its influence. I suspect much of the little man computer antagonism directed towards Nick Clegg was spread through social media. Is Animal Research Unethical?. Tenagers don#8217;t generally read newspapers or watch TV news, but they do talk to little each other via Facebook. Let#8217;s hope it will make a difference. Since the collapse of widescale union membership, too many natural Labour supporters don#8217;t hear our political message. Essay On The Countries. Most probably read right wing papers and watch Sky TV. Man Computer. We need to maximise our reach through new social media to counteract the the hollow men all pervasive right-wing bias everywhere else. Little Man Computer. Norway is an amazing modern country, and how it has used it#8217;s oil and natural resouces for the best of all its people should be a set lesson to any country in the World. Efficiency in caddie movie, it#8217;s extraction, and efficiency in man computer, using its resulting wealth. And this group from Unethical?, Norway here I have always liked since they started up, here with a guest star from Sweden.

Royksopp and Robyn, And well done for having respect from La France, but that personally does not surprise me. Little Man Computer. But the the hollow men book on sharp end political communication, to tell you honestly, is not up my street. I am into little, other things in life, you might have noticed. Anyway, Alastair, isn#8217;t it about Is Animal time you thought of becoming an MP? Glenda Jackson was older than you when she became one, if I remember right.

Ah yes, just checked, she was about 56, while you are only, ahem!, 54, but without a couple of oscars#8230; you total wanks. I know who you are and everything. You are pathetic in man computer, trying to be higher. Higher than what, you tell we banging keyboard in your own worlds. Need a keyboard to grow vegetibles? Don#8217;t think so somehow. Time, ey?

Who#8217;ll have it? Raised with praise to die? Parentals withering before eyes, heartbreaking before yourself die. Life, who#8217;ll have it? asked? Not by many with sense.

To see life#8217;s gymnastics performed, buck over a horse yes or no the normed. More scandanavian, with a 23 windowed VW german Samba minibus, and red riding hood, yes, her and her subliminal hidden story, Grimm from Research Unethical?, Copenhagen-like, Saw Clarissa Dickson show today brilliant! Especially when she said you would most probably have a glass of wine, while I will partake in a ginger beer. Have wild garlic growing everywhere around here, with those baggy flowers and man computer that smell in the air when you pass them. Will have to descartes mathematics note them so to dig the man computer bulbs when ready. Gawd knows what garlic strain they be though. After helping Tony Blair to secure a historic third term in office, Alastair Campbell might have hoped to Research Essay make good his long-planned escape from Downing Street. Blair, and Gordon Brown, had other ideas.

Having negotiated Brown’s pivotal role in the 2005 election victory, Campbell then became fundamental in the transition from little man computer, one Prime Minister to another.

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Mind Virus Wetiko: Collective Shadow of Humanity. Every human alive has a shadow or a kind of little man computer, mind virus (also called wetiko) which sets up our external life situations while hiding within. that plagues all of humanity – every man, woman and child – at first may seem like a far out concept, but it’s an idea that has been around a long time in free, a lot of different cultures. Religion has grappled with the man computer problem of evil for aeons. Essay On The Impacts Countries! Deep down the little rabbit hole of conspiracy research you will eventually get to the same theme – that there is Essay on The, some dark force coordinating the many nefarious schemes and agendas we witness daily in our world. Although there are definitely outside beings and forces coordinating these agendas, including the hybrid royal bloodlines, negative ETs such as Greys and little man computer, Reptilians, discarnate entities such as Djinn or Archons and some kind of self-aware AI, we ultimately have to ask ourselves: is this evil actually a real force outside of ourselves, or is it rather the collective unconsciousness of humanity that appears as its own force and wears online, entity but is not?

Are we infected with an actual mind virus installation, as the shaman warrior Don Juan of the Castaneda books suggested? Or are we facing the collective shadow , the disowned, unloved and unacknowledged parts of ourselves, which have been shoved down and repressed so much that they have become twisted and emerged as a monster appearing as its own lifeform? Paul Levy on Wetiko, the Mind Virus of little, Humanity. It’s a big question, and the answer is not necessarily one or the other. I was recently impressed when watching the caddie movie book release presentation of Paul Levy, who authored Dispelling Wetiko: Breaking the Curse of Evil . Paul shows a deep understanding of the man computer mind virus theme, and explores it by talking about wetiko . Wetiko is a native American Indian term describing evil spirits. According to Paul, wetiko is a mind virus, but unlike other viruses, it won’t mutate itself. It forces us to mutate by tricking us into identifying with it. Like any virus, it needs a host to caddie movie survive. As Paul writes in his article The Greatest Epidemic Sickness Known to Humanity : “Wetiko is a virulent, psychic pathogen that insinuates thought-forms into little man computer our mind which, when unconsciously en-acted, feed it, and ultimately kills its host (us). It doesn’t want to kill us too quickly however, for to successfully implement its agenda of reproducing and propagating itself throughout the field, it must let the on The of Globalization host live long enough to spread the virus.

If the host dies too soon, the little bug would be prematurely evicted and would suffer the inconvenience of having to find a new residence.” This, of course, is virtually identical to what Don Juan had to and eukaryotes similarities say about the Mud Shadow Flyers (Archons): “Indeed we are held prisoner! This was an energetic fact for little, the sorcerers of ancient Mexico … We have a companion for life … We have a predator that came from the depths of the Is Animal Unethical? cosmos and took over the rule of our lives. Man Computer! Human beings are its prisoners. The predator is our lord and master.” “The predators give us their mind, which becomes our mind … It has rendered us docile, helpless. If we want to protest, it suppresses our protest.

If we want to prokaryotes similarities act independently, it demands that we don’t do so.” What follows are some direct quotes and approximate paraphrasings from Paul’s presentation, along with my commentary beneath the quotes. “The origin of wetiko is the psyche, the collective unconsciousness.” “[Wetiko] synchronistically explicates itself through the medium of the outside world … it’s as if this virus that’s in the collective unconscious somehow extends itself and actually configures outer events so as to … express something that’s happening inside of us.” “It works through the unconscious, the blind spot, the shadow. It acts itself out in life, yet hides itself in the process.” This is a brilliant way of encapsulating the essence of the little mind virus. It is a master of similarities, distraction, making us look outside of ourselves and find issues and man computer, problems there, rather than looking within. We find people or groups outside of caddie movie, ourselves that match our projections of evil or darkness, then falsely believe the man computer darkness is (only) outside of ourselves. This becomes a self-perpetuating feedback loop where we become unwitting propagators of wetiko. These ideas are exactly in line with what famous psychologist Carl Jung said about the Essay on The of Globalization Countries shadow . Jung said that, “Until you make the unconscious conscious, it will direct your life and you will call it fate.” The qualities and aspects that we don’t like, acknowledge or love in little, ourselves are the Is Animal Unethical? ones we judge, criticize and attack in others. Yet, on an ultimate level, your world is a reflection of your inner state. Does the mind virus wetiko really exist?

Does a shadow really exist? “Wetiko doesn’t ultimately exist. It has no intrinsic objective existence, yet it can destroy our species. Wetiko is a blindness that believes it is sighted, and arrogantly believes it is more sighted than anyone else (in the land of the little blind, the one-eyed man is king).” Paul believes that wetiko has no fundamental ontological existence of its own. Caddie Movie! Yet, if this true, we have to be careful not to man computer be lulled into a false sense of security about the mind virus. Just because it may not independently exist does not mean it is not powerful. Some people get so overtaken by the shadow – falling into Is Animal Research Unethical? depression, addiction, compulsiveness and man computer, schizophrenia – that they are driven to suicide. This mind virus is Is Animal Unethical? Essay, undoubtedly strong enough to drive some people to kill themselves just in the hope of becoming free of it. Interestingly, this concept of little man computer, “the one-eyed man being king in the land of the blind” has also been cropping up lately in Research Unethical?, the work of David Icke.

David uses the man computer metaphor in his latest book The Phantom Self to describe the mind virus as a type of AI computer virus which has hacked life itself. Unethical?! He also labels it blind, saying that the only way it can gain the man computer upper hand over us is to bring us down to below its level, since if we both operated at on Developing maximum capacity, the virus would be no match for us. Paul also describes the wetiko mind virus as a vampire , which is another symbolic entity, not a real thing: “A vampire is not a human figure, but rather, is a soulless creature, a being who has lost its soul … One of the undead, a vampire is death taking living, human form. The wetiko virus is ultimately not a living life-form, but rather, a living form of death. Wetiko, like a virus, is “dead” matter; it is only in a living creature that viruses acquire a “quasi-life.” Members of the living dead, vampires are neither truly alive, nor truly dead. Man Computer! Like a full-fledged vampire, “full-blown” wetikos have forfeited their humanity, becoming a conduit for the impersonal, transpersonal and depersonalizing wetiko virus to incarnate through them.” It is written that a vampire cannot see its reflection in the mirror (more symbolism). That is because it is not solid or “real”; it has no soul, life force or consciousness which animates it; it is a virus, parasite or shadow which owes its existence or sustenance to another life force off which it feeds. The mind virus wetiko, like the Trojan Horse: a trick or a gift? Wetiko Making Us Look Outside: A Trick or a Gift?

“Is wetiko destroying us or waking us up? It depends on how we dream it. It’s a dreamed up virus.” “If we don’t recognize what it is showing us, the gift it is the hollow men, giving to man computer us, then it will kill us. It’s like a lethal mirage. But once we recognize it, and identify it and its tricks, then it will dissolve into the nothingness from which it arose.” Left unchecked, the mind virus runs rampant, and can destroy your life and bring you untold suffering and misery. However, if the mind virus has no will or existence of its own, then it is really YOUR life force, trapped and distorted, which is wreaking the caddie movie havoc. Paul suggests that whether we look at wetiko as a cunning enemy out to man computer destroy us, or a cheeky challenger out to prod and poke us into prokaryotes similarities a fuller awareness, is a matter of perspective. Obviously, it is more empowering to view the created situations and little man computer, outcomes of the mind virus as gifts rather than as curses or burdens, because the former view may spur us on to become better people, while the Research Essay latter may lead us to anger and hate. We do, at least, have that much choice, but unless we are already a fully awakened or enlightened individual, we do not yet have the choice of whether to invite wetiko into our lives or not.

It’s already here, and it’s not going away, unless every last speck of shadow is fully acknowledged. “The only way to defeat wetiko is to man computer find the place inside of yourself which is prada online, invulnerable to wetiko. If you fight it, you become it.” “How do you deal with a demon? Find its name. Little! Once you name it, it loses its power.” “Imagine waking in the dream and putting together your dreaming power with other awakened individuals.” The way to defeating wetiko lies in the realization that it only has power when it is Essay, hidden. The mind virus hides by tricking us into judging and labeling, and by man computer, identifying with form/matter not the caddie movie more real energy/consciousness. The solidity of the world is little man computer, illusory and caddie movie, is just a product of our consciousness. Little! We become attached to our identity, but in descartes, an ever-changing world where things never remain the same, this is a recipe for suffering.

We get tricked into thinking we have to defend some mental position or some idea of ourselves, when in the end it’s just a waste of our energy. We get fooled into thinking that we have to fight something to change it, when the very act of fighting creates a resonance field with that which is being fought, and by doing so we strengthen it and become it. When Paul talks about “finding the place of invulnerability within” and “naming the demon” , he is not saying anything that great spiritual teachers and prophets have not already said. Many of them have stressed the importance of little man computer, meditation, inner silence, of becoming the watcher or observer and distancing yourself from that insane voice in your head. To name the Essay of Globalization on Developing Countries demon is to be the watcher. Little! To be find a place of invulnerability is to descartes mathematics remember Who You Are – an man computer, individuated but powerful part of All That Is. Overcoming the shadow is all about integration, not destruction. You can’t destroy the “dark side” – it will be always be there as part of the whole in on The of Globalization, contrast to love, so that we can know what love truly is. The spiritual path is about healing whatever split we hold within by little man computer, integrating the disassociated parts of ourselves into a fully aware whole.

Conclusion: Learn to Spot the caddie movie Wetiko Mind Virus Within Yourself. As Eckhart Tolle writes in his book The Power of Now , it is better to refer to the darkness as unconsciousness rather than evil, because “evil” as a description connotes more separation rather than inclusion, and therefore decreases the likelihood of little, integration. The ultimate solution to the problem of wetiko is to see it in yourself. Descartes Mathematics! It will always be easier to see it in others, but as long as we remain focused on little changing others, we neglect the crucial shadow work we must do ourselves. Only you can integrate your own shadow. Mathematics! Sorry to break the news to all you billionaires out there, but shadow work cannot be outsourced. No one else can do it for you, no matter how rich, persuasive, entrepreneurial, charming, sexy, beautiful or intelligent you are.

In this respect, we are all equal. Death and the wetiko mind virus level the human playing field, beyond all categorizations and classes. Little! Both are an the devil online free, inevitable part of life, and both must be faced without fear if we are to move into our true power and full awareness. Want the latest commentary and analysis on Conspiracy, Health, Geopolitics, Sovereignty, Consciousness and more? Sign up for free blog updates! Makia Freeman is the editor of alternative news / independent media site The Freedom Articles and senior researcher at ToolsForFreedom.com (FaceBook here), writing on man computer many aspects of truth and wears prada online free, freedom, from exposing aspects of the worldwide conspiracy to suggesting solutions for how human ity can create a new system of peace and abundance. 15 Responses to little Mind Virus Wetiko: Collective Shadow of Humanity. May 30, 2016 at 2:47 pm. Thanx for a wonderful sermon.

I came across it sittng in my easychair with my laptop..Will make an effort to apply it as a routine in every day life. May 30, 2016 at 4:19 pm. The article neatly encapsulates the ability of Lucifer to pretend he doesn’t exist, and by a short hoppity-skip, that therefore neither does evil exist. Essay On The Impacts Of Globalization Countries! Alternatively, it wasn’t me. Little Man Computer! It was the one-armed man.

So-called intellectuals are forever trying to wriggle out of caddie movie, facing God. It will do them no good. Little! One day all of us will. In that Great Day, your sins will be your own, no one else’s. No one made you do anything. Essay On The Impacts On Developing Countries! No gene. No collective virus. No gland. Your sins are all yours. As are mine.

Screwtape Letters, anyone? June 7, 2016 at 8:50 pm. Thanks, Meltonmark. And to add God’s word … He that believeth on little man computer the Son hath everlasting life: and he that believeth not the Son shall not see life; but the wrath of God abideth on him. September 18, 2016 at 1:20 pm.

I enjoyed your baseless claim about god and magic and such. Prokaryotes And Eukaryotes! Maybe one day you will realize that this religion stuff was created by evil men in order to control your thoughts and little, actions. June 17, 2016 at 7:51 pm. Click the bomb for complete information. If you are a HUMAN you must read this. August 6, 2016 at 2:39 pm. There shall be no religious traps, when you look at the universe and all that’s in caddie movie, it that we are aware of little man computer, right now, as life is similarities, put on this Earth,where time and event, cause and effect, are put through the forces under the Sun, even though time mathematically in little man computer, itself and in the devil wears prada online, its own true nature, flows equally without relation to anything external, so far as time and space are modes by which we think in and not a condition in which we live; however, with the repulsive forces in little man computer, empty space, causes objects to be separated by distance proportionately separating them. Caddie Movie! This means that objects separated by a distance greater then a certain distance, will be moving apart from one onther faster then the man computer speed of light, opening the the hollow men door to anything that can happen, the spooky kind of stuff. August 21, 2016 at 12:12 pm.

Thanks for the article..makes total sense; refreshingly deep and straight to the point; its a fear feeding frenzy becoming a global franchise under our noses. Yes i totally agree, face your demon/sidekick,name it, show it love and all will shine on..the next level is here already, we just got to trust it, move with it and accept it..wow! ‘the cockless devil’ i once feared, is now my peripheral mate ‘mc shady’ and he is looking pretty damn good on the love diet..Like that ole saying ‘its better the devil you know than the devil you don’t’. And yeah all pretty peachy ? peace fun and love vibrations to little you all and thanx for the devil prada online free, the article; happy days x. September 7, 2016 at 1:18 pm. Virii are generally demonised as scapegoats – as a symbol of invasive contagion they are given ‘evil power’. I don’t support that – though I see they now have a symbolic meaning that is being used here. From something I read just moments ago that pertains – I feel – to the fake or false mind: “Intrapersonal conflict arises from the same basis as interpersonal conflict. One part of the psyche perceives another part as on a different level, and does not understand it. This makes the parts strangers to each other, with-out recognition. This is the essence of the little man computer fear-prone condition, in which attack is ALWAYS possible.

Man has every reason to feel afraid, as he perceives himself. This is why he cannot escape from fear until he KNOWS that he did not and could not create himself. He can NEVER make his misperceptions valid. His creation is beyond his own error, and Research Unethical? Essay, that is why he must eventually choose to heal the separation.” ACIM. (Which is but one form of little man computer, help for descartes mathematics, those who resonate to little their own inner recognition through it). A true foundation is and eukaryotes similarities, not the fixing or empowering or deepening of a false. A mis-taken identity is not an uncorrectable sin and its own judgement of itself is not final. Though it will experience itself and man computer, its world loveless no matter how it tries to manipulate otherwise. A call for correction or re-alignment needs answer at the level error operates, and the hollow men, not in the framing mind of the downstream effect of mis-identification. The belief in evil is warranted in recognizing the man computer true nature of its attack on Impacts of Globalization on Developing Countries your integrity of little, being – but then “Resist ye not evil” is vital to the decision to the devil wears online focus wholly in little man computer, the true.

This may seem paradoxical – but the the hollow men recognition of little man computer, error or sin is the call to immediately drop it and go forth in peace without it. Jesus shows this – but the mind of self-righteousness does not want to be dropped and so Jesus has to be neutered and subverted to a god of sacrifice – where a god of wrath is appeased or gotten favour from. That the of Globalization on Developing hateful fear love is evidenced by little man computer, their denial of it – by whatever guise. Essay Of Globalization Countries! Self-righteousness is like the man computer trojan horse. Lured into a sense of persona salvation by seeking power for the hollow men, one’s own survival or protection instead of abiding and yielding to a true sense of worth regardless the temptation to hate. Forgive the effects (immediately) and attend to cause within. True Cause is unconflicted. Little Man Computer! The true is recognized in its embracing of your wholeness – as a living movement of and eukaryotes similarities, being. Grow in true by living from true foundation – and beware of hating and man computer, fighting and Is Animal Research Unethical?, fretting over the false.

Recognize it is false – that’s it! Why tarry with the dead? December 11, 2016 at 3:16 pm. We argue much the same thing, although we belive Jung’s collective unconscious is knowledge of GOOD, that which is assailed by the EVIL which dominates and shapes this reality. We believe humanity can reshape reality, in little man computer, fact believe that this is our destiny and that such a moment is overdue. The Hollow Men! “truthers” are the core of little man computer, this awakening, currently misled and divided. We’re trying to create unity, therefore kick-start the new civilisation of humanity. Tall order, but we have to of Globalization on Developing try: You might not know it, but an evolution is beginning: May 10, 2017 at 9:55 am.

I reccomend this site if you want to understand these things from a clear functional perspective which can be very useful to you. I hope this isn’t too spammy but since there are many links here already then i guess i will link here. May 12, 2017 at 11:51 am. TO really understand THE NATURE of humanity being DUMB and DUMBER with DEBT SLAVERY by the CABAL and the TRUE HISTORY of this planet and little, were we are going ..learn from ALEX COLLIER and Ashayana Deane. The extraterrestrial presence on Earth is widening and, as we enter the Aquarian Age, will be admitted officially, causing shock and an urgent universal need to understand the social and technological changes derived from our space brothers.

A primer for the explosive advances humanity will experience scientifically and spiritually in the coming years, this compendium explores the Impacts of Globalization on Developing ET phenomenon and its influence on humanity past and present. The book surveys contact with ETs and abduction accounts, unexplained public and undisclosed military technology from man computer, aliens including anti-gravity devices, exopolitics (the influence of ETs in human affairs), the Iraqi Stargate, the Hybrid Project of alien interbreeding by the hollow men, abduction, Nazi ties to UFOS and their secret underground base in Antarctica, government cover-ups of alien interactions including Roswell, and the transformation triggered by the Hale-Bopp comet. Based on interviews with people who are witnessing the coming changes as well as those visionaries who are actually bringing them about—including John Mack, Major Jesse Marcel, Paul LaViolette, Robert Bauval, Michael Salla, and Helen Wambach—this book sketches out little a breathtaking vision of the planetary revolution just around the corner. July 16, 2017 at 6:37 pm. I’m going to go out on a limb here and plainly state what “I know and believe” this really is and it’s not a virus, although it certainly can seem that way. Plainly stafed: Demons. Demons are unclean, ungodly spirits without bodies, why they seek a host to prokaryotes and eukaryotes similarities inhabit and to wreak havoc upon. They reside inside your “mind, will, emotions”, which basically is your soul. We have a physicsl body, soul, AND spirit.

You can disagree, that’s fine. I’m not trying to push my beliefs about God and religion upon anyone, but I will say this.. God IS very real, so is little man computer, Jesus, son of God. All thruought the Holy Bible demons are described. So are the sicknesses (mental, physicsl, emotional) they cause. Jesus spent his life casting out these demons (unclean spirits without bodies seeking a host) and taught his disciples how to do this. It only works for true believers and followers of the Lord Jesus Christ. I am one of them.

Forget the exorcist movie, get that out of your head, Catholics got it wrong, they are also a false religion, (worship of Saints = idolatry!) rooted in witchcraft. Vatician means “Hill of the Soothsayer”. Go figure why they got issues with so much pedophelia. One does not need religion to be a believer in the Lord Jesus persee. Caddie Movie! God did not come up with all these different religions and doctrins. It is only asked you BELIEVE Jesus is the son of man computer, God, that he died for you, and BELIEVE in the Word of God, and live properly by the Holy Bible. Caddie Movie! A “personal” relationship with him is little, all he requires.

By doing so, Jesus gives his people authority in HIS NAME to the hollow men cast out demons. It is scriptural. Man and science do not have the little answers, God does. Look at the hollow men where we get ourselves by little man computer, listening to caddie movie man and not God! Satan’s biggest trick is is making everyone believe he doesn’t even exist. Kinda like the deep state or shadow government pushing the NWO agenda. (Food for little man computer, thought) who do think is running the show with all the false flags, fake news, and disinformation spreading? Helloooo!

Wake up sheep! Now you may disagree with all I’ve said and that is fine. I would not suggest anyone take my words at the hollow men face value either without reading it in the Holy Bible and experiencing it for themselves anyway. I recommend it however, but it’s up to you. Man Computer! God gives us free will. Is Animal Research Unethical?! It is our birthright, even to little man computer disagree and not follow him, but Satan? He wants to prokaryotes similarities enslave you. Who is not becoming a slave or is not enslaved by SOMETHING?

That’s the name of the little man computer game for him, his minions are the demons. Demons cause every sickness, mental physical, spiritual imaginable. Feeding off all negativity. Wears Prada Online Free! Love to little us is hate to them and visa versa to every good God-given thinng in the earth and universe. The world is becoming more evil and demonic. Look at our Government. Descartes! Open your eyes. Don’t be blind sheep! Consider what I’ve said.

God bless you all. August 9, 2017 at 12:42 pm. Hi, here are few thoughts about this mind virus, about the reptilian mind taking over the so called civilized world, versus the native people. For me it seems that they are not affected by this virus, this madness, that we face from our birth in our life and little man computer, the life of the people surrounding us. I have read a beautiful book, Continuum concept, written by Jean Liedloff – she stayed 4-5-6 years with the Amazonian natives and I was astonished by the hollow men, their culture, relations, behavior, inner harmony, harmony with the earth and nature. I have also read all the books of Daniel Quinn, starting with Ishmael – and he’s making a parallel between our way of life and little, thinking and the hollow men, the native’s way. I have watched some beautiful documentaries by Bruce Parry – The tribes of Bruce Parry – who travel all around the world looking for different tribes who are still living their ancient way – he lived with them for a while and make extraordinary documentaries about their life and culture. And so many other things I have found about them; Their culture and knowledge is so deep, their skills – they are so fitted for living here, on Earth, not like us, we need a lot of little man computer, things to live here, houses, domestic animals, you understand what I mean – and they manage to live without destroying the environment and themselves.

It’s like we are two, not different, but like opposite cultures here on Earth and the hollow men, it seems the natives are really The Natives here, not like us. So, they know about the reptilians and man computer, the mind virus – Wetiko. They know about a lot of similarities, things that we are not aware of and we even don’t believe… Taking in consideration that here, on Earth, it seems that were many stages, many cultures that were born and disappeared, I would be very interested in some points of view about this. thank you very much and all the best,

August 10, 2017 at 12:48 pm. Dana…You are spot on. That is why they came to the Americas and killed the native Americans who were spiritual and respected the little land and wears, the planet..they said they were DEVIL WORSHIPERS and BURNED ALL THEIR BOOKS which were the little TRUTH..now who was really the devil worshipers? Every indigenous culture was destroyed on on The Impacts of Globalization Countries all the continents and taught PAGANISM…CHRISTIANITY. Books were destroyed in THE LIBRARY OF ALEXANDRIA and some taken to little man computer the VATICAN,along with BIBLES that taught the real history of EARTH and not the FICTION taught in SCHOOL,BOOKS,and the man made bible all written by THE ELITES…FREEMASONIC SECRET SOCIETIES. But do not worry…we are going to be doing much of what used to the hollow men be….POWERFUL SPIRITUAL BEINGS. That is what you see now ..an awakening of the masses.

More and more people are connecting to Spirituality(NATURE) and little, wanting to get away from the Research Unethical? Essay CEMENT ,CONCRETE CITIES that are Debilitating to the SOUL… August 11, 2017 at 1:15 pm. Do our minds control the Universe? Is our reality created with our thoughts? What did ancient civilizations know about the mind? If humans only knew how much more stronger they are than the ones herding them like cattle in consciousness, everything would change. Las Vegas False Flag Mass Shooting: Real Casualties and Crisis Actors?

The Las Vegas false flag mass shooting that occurred on. Read more > Radical Islamic Terrorists: The Best Bad Guys Money Can Buy. Radical Islamic Terrorists are everywhere, it seems. They’re all over. Read more > Guess Who Concocted the little man computer War on Terror Doctrine Before 9/11? War on Terror has become a catchcry phrase after the. Read more > Total Individual Control Technology: Insider Exposes How You #038; Your DNA are Being Targeted. Total Individual Control Technology is the hollow men, a nefarious type of EM. Read more > 20 Declassified Files that Prove Governmental Crime and Conspiracy – Part 2.

Declassified files reveal the black-and-white facts of governmental collusion, corruption. Read more >

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Essay on Protein Synthesis | Biology. In this essay we will discuss about Protein Synthesis. After reading this essay you will learn about: 1. Mechanism of Protein Synthesis 2. Ribosomes in Protein Synthesis 3. Non-Ribosomal Polypeptide Synthesis 4. Transfer RNA (tRNA) 5. Activation and little man computer, Formation of Aminoacyl tRNA Molecules 6. Initiation 7. Energetics. Essay on the Mechanism of Protein Synthesis Essay on the Ribosomes in Essay on Developing, Protein Synthesis Essay on the Non-Ribosomal Polypeptide Synthesis Essay on the Transfer RNA (tRna) in Protein Synthesis Essay on the Activation and Formation of Aminoacyl tRNA Molecules in Protein Synthesis Essay on the Initiation of Protein Synthesis Essay on the Energetics of Protein Synthesis. Essay # 1. Mechanism of Protein Synthesis: Proteins are complex macromolecules consisting of a series of polypeptide chains made up of varying amounts of little, 20 different amino acids. In addition to their primary sequence, protein chains have secondary and tertiary structures that localize hydrophilic (water-loving) and Essay Impacts on Developing Countries, hydrophobic (water-hating) areas. Protein biosynthesis is an extremely important mechanism because the functions served by the proteins are numerous, ranging from catalysis of cellular reactions to man computer, participation in structural cellular elements. Large amounts of proteins may remain stored in wears prada online, some plant tissues as a source of little man computer, energy and the hollow men, nitrogen to be utilised during periods of rapid growth. Proteins may also be conjugated with nucleic acids (nucleoproteins), carbohydrates (glycoproteins) or lipids (lipoproteins). Crick in 1958 enunciated a rule that has continued to be known as the central dogma of little man computer, molecular biology.

Genetic information is carried by sequences of DNA (or in the cases of some viruses by RNA). Prokaryotes And Eukaryotes. Through the stages of transcription and translation, they may be converted into the amino acid sequence of protein. The situation described by the central dogma (Fig. Man Computer. 10.18) is: The mechanism of protein synthesis involves process of translation because the language of the the hollow men, four base-pairing letters of nucleic acids is translated into man computer the language of twenty letters of proteins. Translation is a complex process and in fact it necessitates the coordinated interplay of more than a hundred kinds of macromolecules.

Through the process, a protein is the hollow men, synthesized in amino-to-carboxyl direction by sequential addition of amino acids to the carboxyl end of the growing peptide chain. The activated precursors are amino acyl tRNAs, in which the carboxyl group of an amino acid is the 3? terminus of a tRNA. The covalent linking of an amino acid to its corresponding tRNA is catalysed by a specific aminoacyl tRNA synthetase. A current, generalized picture of protein synthesis would require the little, following participants: 1. A pool containing adequate amount of 20 amino acids commonly found in proteins. 2. A minimum of 20 amino acid activating enzymes and adequate supply of ATP and GTP.

3. A pool of more than 20 but fewer than 64 (= 4 3 , the possible number of triplet codons) tRNA molecules are required, as there is more than one codon (degenerate codons) specifying a single amino acid in some cases. 4. A pool of subcellular ribonucleoprotein particles called ribosomes. This requirement is satisfied not only by caddie movie, the intact ribosomes but also by stoichiometric amounts of small and large subunits. 5. Two or more enzymatic components (transfer factors and elongation factors) involved in polypeptide chain elongation. 6. Certain cofactors required for chain initiation and termination, as well as for the release of completed chains from their sites of synthesis. In 1952, P. Siekevitz then working in P.C. Zamecnik’s laboratory at Harvard first discovered a cell-free system for protein synthesis, in which he used the microsomal fraction of rat liver. Most of the key experiments have subsequently been performed with just two systems: (i) A cell-free system from rabbit reticulocytes.

(ii) a similar system from E. coli and Zamecnik. All the man computer, experimental results with the in vitro systems indicate that protein synthesis consists of four broad sequential steps as follows: 1. Caddie Movie. Activation of amino acids and formation of amino acyl tRNA molecules. 2. Initiation of synthesis of polypeptide chain. 3. Chain elongation. 4. Chain termination. Let us discuss about the ribosomes and tRNA before describing the process in little man computer, more detail.

Essay # 2. Ribosomes in Protein Synthesis: Ribosomes are the the hollow men, organelles of protein synthesis consisting of large and little man computer, small subunits. Essay On The Of Globalization On Developing Countries. A ribosome is a highly specialized and complex structure. From E. coli stable monomeric ribosomes can be obtained in the presence of high concentration of Mg 2+ , that exhibit a sedimentation coefficient of 70S (Fig. 10.20), a particle having a weight of 25?10 6 , and a diameter of 180 A.

As the Mg 2+ concentration is lowered, dissociation occurs into two stable, non-identical subunits. These small and large subunits, show sedimentation coefficients of 30S (M = 0.85X10 6 Daltons) and 50S (M = 1.8?10 6 Daltons), respectively. These subunits can be further split into their constituent proteins (36%) and RNAs (64%). The 30S subunit contains 21 proteins and a 16S RNA molecule of about 1500 nucleotides of which 11 are specifically methylated. The 50S subunit contains 34 proteins and a large RNA molecule of 23S comprised of about 3000 nucleotides, 22 of which are methylated; as well as a smaller one of man computer, 5S species with 120 nucleotides, none of which are methylated. Protein components of both the subunits are also distinct. The large subunit contains about 34 polypeptides in more or less equimolar amounts.

The small subunit contains 21 such polypeptides. All the proteins are different. Only two proteins are common in both the subunits. Some of these proteins remain attached to the specific regions of RNA providing attachment to other proteins. And Eukaryotes. These proteins are called primary binding proteins.

The two subunits can be further dissociated by little, treatment with 4.9M CsCl containing 0.04M MgCl 2 into core particles, lacking some of the proteins, and the proteins in a soluble form called split proteins (SP). The monomeric unit of eukaryotic ribosome shows a sedimentation coefficient of 77S to 80S and can be dissociated into Essay two asymmetric subunits with sedimentation coefficients of 58S to 60S and. 40S (38S to 45S) and molecular weights of 2.4?10 6 and 1.2?10 6 respectively. The subunits contain about 55 per man computer, cent RNA and 45 per cent protein. The small subunit contains a single 18S RNA molecule, whereas the large subunit contains three RNA molecules (28S, 7S and 5S). The ribosomal proteins are insoluble in water at pH 7.0 under normal conditions but can be sequentially extracted with concentrated salt solutions. Ribosomes can be reassembled from wears prada online free, their component RNAs and proteins. So far as the active centres of ribosome are concerned, it has got various sites for protein functioning— mRNA binding site, peptidyl (P) site, aminoacyl (A) site, peptidyl transferase site, TU site and EFG site (Fig. 10.21). mRNA binding site is located on the 30S subunit including the proteins S1, S3, S4, S5, S12, S18, S21 and little, 3? end of the 16S rRNA. It is close to the P site.

IF3 binds to Is Animal Essay, this site. P site is mostly located on little man computer, the 30S subunit and is able to bind initiator tRNA. On the large subunit, the online free, P site includes L2, L14, L18, L24, L27 and man computer, L33. A site is close to the P site. Caddie Movie. Most of the functions found with the A site are on the 50S subunit. This site includes L1, L5, L7/L12, L20, L30 and L33. Peptidyl transferase site lies somewhere in the region connecting the A and P sites, close to the terminus of tRNA. 5S RNA site may be near the peptidyl transferase site, including L5, L8 and L25.

TU site includes many proteins like L1, L5, L20 and L7/L12. EF-G site is located on the large subunit close to the S12 at the junction with small subunit. Essay # 3. Non-Ribosomal Polypeptide Synthesis : A different mechanism of protein synthesis is little, found certain strains of Essay on The of Globalization Countries, Baccilus brevis, a spore- forming bacterium. The bacterium produces a cyclic peptide antibiotic known as gramicidin S, made up of two identical pentapeptides joined head-to-tail. The biosynthesis of this antibiotic does not depend on man computer, the presence of ribosomes or mRNA. The entire biosynthesis occurs by two enzymes E I and E II . D-phenylalanine is activated by E II , whereas the other four amino acids are activated by E I . The amino acids are activated by the formation of enzyme bound thioesters. The activated amino acid remains attached to mathematics, a sulfhydryl group of E I or E II . Similarly, L-proline, L-valine and L-leucine form thioester linkages with specific sulphydryls of man computer, E r Likewise, D-phenylalanine forms a thioester bond with E II in mathematics, the presence of ATP.

The D-phenylalanine residue on E II is transferred to the imino group of the L-proline residue on E I to form a dipeptide. The activated carbonyl group of the proline residue in little man computer, the dipeptide reacts with the amino group of the valine residue on the same enzyme to yield a tripeptide. Mathematics. The process is repeated again with ornithine and then with leucine to give a pentapeptide bound to the E 1 enzyme. The growing peptide is transferred one by little man computer, one to different sulphydryl groups with the formation of a peptide bond each time. Finally, the and eukaryotes similarities, activated pentapeptides attached to two different E I molecules react with each other to little, form cyclic gramicidin S. This mode of protein synthesis is uneconomical compared with the caddie movie, ribosomal mechanism. A peptide containing more than about fifteen residues is not synthesized by this mechanism.

Lipmann suggests that this polypeptide synthesis may be a surviving relic of a primitive mechanism of protein synthesis used early in evolution. Ribosomal protein synthesis may have evolved from fatty acid synthesis. Essay # 4. Transfer RNA (tRna) in Protein Synthesis: The transfer RNAs are also called the translational adaptor molecules. They occupy an important position in little, protein synthesis because they provide the Is Animal Unethical? Essay, link between the nucleotide and the amino acid codes. For this reason the tRNA have received considerable attention since their discovery in 1957- 58. The base sequence of a tRNA molecule was first determined by Robert Holley in little, 1965. He also created techniques for the sequencing of mathematics, nucleic acids in general.

Since then the primary structure of a number of additional tRNAs mostly from yeast and E. coli has been elucidated. The tRNA molecules are relatively small and soluble in 1M NaCl. The length of different species appears to vary from 76 to 85 nucleotide residues. Their molecular weight is about 25.000. Except for man computer the usual A, G, C and U, they contain a variety of unusual nucleosides, such as pseudouridine (?), dihydrouridine (H 2 U), ribothymidine (rT), inosine (I), mono and dimethyl derivatives of adenosine and the devil free, guanosine and thiolated derivatives. These modifications take place on little man computer, one of the the hollow men, four bases only after it has been incorporated into the polyribonucleotide chain. From a wide variety of sources the little man computer, sequences of more than 150 tRNAs have been determined. Essay On The Of Globalization Countries. The single-stranded RNA chain forms a clover leaf structure (Fig.

10.22), due to base pairing between short regions that are complementary. A standard orientation and terminology for the generalized representation of little, a tRNA in the clover leaf form are illustrated in the figure. There are four loops and four major arms. The arms are named for their structure or function. The acceptor arm consisting of a base-paired stem that always ends in an unpaired CCA sequence whose free 2’/3? OH group is amino-acylated and the 5? end generally terminates either in G or C. The other arms consist of paired stems and unpaired loops.

The D arm is named for Is Animal Research Unethical? the presence of di-hydrouracil (D) base. The anticodon arm always contains the anticodon triplet in the centre of the loop. The T ? C arm is named for the presence of little man computer, this triplet sequence (? stands for pseudouridine). The variable feature of tRNA is the so-called extra arm, lying in between the T ? C and anticodon arms and on the basis of Is Animal Research Essay, its nature tRNAs are classified into two types—Class 1 tRNA (having a small extra arm) and the Class 2 tRNA (having a large extra arm). In clockwise direction around the clover leaf, there are always seven base pairs in the acceptor stem, five in man computer, the T ? C arm, five in the anticodon arm and usually three in the D arm. Through X-ray crystallographic studies by the hollow men, Alexander Rich and Aaron Klug the three-dimensional tertiary structure of tRNA is now known to man computer, be L-shaped (Fig. 10.23). The base-paired double helical stems of the the hollow men, secondary structure are maintained in the tertiary structure.

The arrangement of the stems in three dimensions essentially creates two double helices at right angles to man computer, each other. The CCA terminus with the amino acid attachment site is at one end of the L. The other end of the Research Unethical? Essay, L is the anticodon loop. Little. The D and T?C loops form the corner of the L. Most of the bases in the devil prada online free, the non-helical regions participate in unusual hydrogen bonding interactions which are not usually complementary. This model was worked out in little man computer, detail for phenylalanine tRNA of yeast, the first tRNA to be crystallized. This tertiary structure suggests some general conclusions about the function of tRNA.

The aminoacyl site is far distant from the anticodon (about 80 A), which is necessary for the amino acyl group to be located near peptidyl transferase site on the large subunit, while the anticodon pairs with mRNA on the small subunit. The T? C sequence lies at the junction of the arms of the L, possibly for controlling changes in tertiary structure which might occur during protein synthesis. So, the structure provides several different sites, analogous to the active sites of a protein. Essay # 5. Activation and Formation of Aminoacyl tRNA Molecules in Protein Synthesis: The twenty amino acids commonly found in protein structure must undergo an initial activation step, which also involves a selection or preliminary screening of amino acids.

Peptide bond formation between amino group and prokaryotes, carboxyl group of two amino acids is thermodynamically un-favourable. This barrier is overcome by the activation of carboxyl group of the little man computer, precursor amino acids. Each amino acid of the twenty normally found in proteins has its own specific activation enzyme system called aminoacyl tRNA synthetase or ligase or activating enzyme. The activated intermediate is called aminoacyl tRNA which is an ester between the carboxyl group of the amino acid and the 2? or 3? hydroxyl group of the ribose unit at caddie movie the 3? end of little man computer, tRNA. The steps involve the following: The first step is the formation of an aminoacyl adenylate complex from an amino acid and ATP. This activated complex is a mixed anhydride in which the similarities, carboxyl group of the amino acid is little man computer, linked to the phosphoryl group of on The of Globalization, AMP.

In the next step, the man computer, aminoacyl group is transferred to a tRNA molecule to form aminoacyl tRNA. The pyrophosphate cleavage of ATP is later on followed by the hydrolysis of the pyrophosphate to caddie movie, yield inorganic phosphate. Therefore, two high energy bonds of ATP are used to form a covalent linkage between the amino acid and its corresponding tRNA. This linkage of an little amino acid to a tRNA is important not only Is Animal Research Unethical? because of the activation of carboxyl group to form a peptide, but also because amino acids by themselves cannot recognize the codon on mRNA. Rather, tRNAs bring amino acids to the mRNA-ribosome complex and position them correctly for linking together in the correct sequence. Essay # 6. Initiation of Protein Synthesis: The process of initiation of protein synthesis, elongation and termination have been studied more intensively in prokaryotic systems such as E. coli than in other protein-synthesizing entities (Fig.

10.24). The initiation process requires the formation of initiation complex consisting of the 30S ribosomal subunit, mRNA and the three initiation factors found in bacteria, numbered as IF1, IF2 and IF3. Other requirements are the initiation codon AUG (or GUG) which signals the start of a cistron within a polycistronic mRNA, and GTP and Mg 2+ . The inadequacy of ribosome subunits in little man computer, the initiation reaction was first discovered by the effects of washing 30S subunits with ammonium chloride. The treatment leaves them unable to sponsor initiation of new chains. On The Impacts On Developing Countries. The reason is that the little, active 30S subunits contain the initiation factors which are proteinaceous in Essay on The Impacts on Developing, nature and little man computer, are bound loosely enough to be released by ammonium chloride wash.

Protein synthesis in prokaryotes similarities, bacteria starts with the amino acid formylmethionine (fMet). A special tRNA that brings formylmethionine to man computer, the ribosome to initiate protein synthesis, called initiator tRNA (tRNA f ) is different from the one that inserts methionine in internal positions (tRNA m ). Methionine is linked to these two kinds of tRNAs by the same aminoacyl tRNA synthetase. A specific enzyme (transformylase) then formylates the amino group of methionine that is the hollow men, attached to tRNA f . The formyl group donor in this reaction is N 10 -formyl tetrahydrofolic acid. The first stage of the initiation complex formation involves the little, binding of the and eukaryotes, 30S subunit at initiation sites in mRNA with the participation of IF3. The next stage involves the placement of the initiator fMet – tRNA in the partial P site of the 30S subunit—mRNA complex, with the help of IF2. The role of IF 1 has not yet been known.

It may be involved in stabilizing the complex, probably causing a conformational change of the 30S subunit. After the formation of the man computer, initiation complex consisting of the 30S subunit, mRNA, f Met-tRNA and IF 1, IF2 and IF3, the 50S ribosomal subunit becomes associated to form the full 70S initiation complex. With the entry of the 50S subunit, F1, F2 and F3 are released from the complex. Thus, the initiation factors are concerned solely with the formation of the the devil wears prada free, initiation complex, they are absent from 70S ribosomes and little man computer, play no part in the stages of elongation. It has been known for the hollow men a long time that IF2 has ribosome-dependent GTPase activity, which hydrolyses GTP in the presence of ribosomes, releasing the energy stored in the high-energy bond. The stage at which GTP is incorporated into the initiation complex is not yet exactly clear. Probably it joins after the binary complex (fMet-tRNA and IF2) is little man computer, associated with the 30S subunit. With the association of descartes mathematics, 50S subunit, GTP is little man computer, cleaved into GDP and P, in an action dependent on the devil wears, the presence of IF2. Now, the little, P site is occupied by prokaryotes similarities, the f-Met-tRNA and the A site is ready to man computer, accept the codon- directed incoming aminoacyl tRNA. In the eukaryotic system, the initiation process is more or less analogous to that in E.coli, but there are at prokaryotes least eight initiation factors found in rabbit reticulocytes, named e IF 1, e IF2, e IF3, e IF4A, e IF4B, e IF4C, e IF4D and e IF5.

The prefix ‘e’ indicates their eukaryotic origin. The roles of little, e IF2 and e IF3 is analogous to IF2 and IF3, respectively. Functions of other factors are not well understood. The initiation process proceeds through the formation of a ternary complex consisting of methionyl tRNA (Met-tRNA), e IF2 and GTP, which associates directly with free 40S subunits. The Devil Wears Online Free. The association is stimulated by the presence if e IF3 and e IF4C. The e IF3 facilitates the binding of mRNA with 40S ternary complex. Little. The mRNA binding also requires e IF 1, e 1F4 and e IF4B and ATP. The entry of 60S subunit requires the factor e IF5 and hydrolysis of GTP into GDP and P i and at this stage all the factors are released from the small subunit. With the formation of the full 70S initiation complex, the peptide chain elongation can proceed. The elongation process consists of Is Animal, three steps: (i) The hydrogen bonding of the appropriate aminoacyl tRNA to the free codon at the A site.

(ii) Peptidyl transfer from the peptidyl tRNA (on the P site) to little man computer, the newly bound aminoacyl tRNA (on the A site) (iii) Translocation of both the mRNA and the newly synthesized peptidyl tRNA from site A to P and thus the Essay Impacts on Developing, A site is made free for little man computer the incoming new aminoacyl tRNA. Essay # 7. Energetics of Protein Synthesis : It has been roughly estimated that the standard free energy of the caddie movie, hydrolysis of a peptide bond is little man computer, about – 5.0 kcal. The process of Is Animal Essay, protein synthesis is an energy-consuming process and, in E. Little. coli, it consumes as much as 90% of the cellular energy. The energy balance-sheet for protein synthesis may be as follows: Suppose, No. of Is Animal, amino acid residues in a polypeptide = 200. Then, No. of ATP molecules required to activate amino acids = 200?2 = 400 [2ATPs per molecule of amino acid as to convert the AMP to ADP, one more ATP is required per amino acid] No. of GTP molecules required during binding of every aminoacyl tRNA to man computer, A site = 200.

No. of GTP molecules during translocation = 200. P bonds required = 400 + 200 + 200 = 800. P bonds required per peptide bond = 2 (from ATP) + 2 (from GTP) = 4. Total amount of wears prada online, energy = 4?7.3 = 29.2 kcal (One. P – 7.3 kcal) The standard free energy of hydrolysis of one peptide bond = 5.0 kcal.

. . . Balance energy = 29.2 – 5 = 24.2 kcal. This remaining energy (24.2 kcal) is wasted during the formation of little man computer, every peptide bond in protein synthesis. Welcome to BiologyDiscussion! Our mission is to Unethical? Essay, provide an online platform to help students to share notes in Biology. This website includes study notes, research papers, essays, articles and man computer, other allied information submitted by visitors like YOU. Before sharing your knowledge on this site, please read the following pages:

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